Measuring LRRK2 Levels in The Ventral Midbrain in a G2019S mutant LRRK2-associated Parkinson’s Disease Mouse Model
Title
Measuring LRRK2 Levels in The Ventral Midbrain in a G2019S mutant LRRK2-associated Parkinson’s Disease Mouse Model
Creator
Samlian, Miranna
Abstract
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the loss of motor control and dopaminergic neurons in the substantia nigra pars compacta region in the ventral midbrain. Only 5-10% of PD cases are familial, with the most common dominantly-inherited gene being LRRK2. LRRK2 holds a high potential as a PD biomarker due to its hyperactive kinase function associated with idiopathic PD and mutant LRRK2-induced PD, where G2019S is the most common pathogenic mutation.
Methodology: In-house Sandwich ELISA was used to measure total LRRK2, flag-tagged LRRK2, and
phosphorylated LRRK2 levels in the ventral midbrains of mice 3-weeks post-injection with helpful adenovirus vectors to induce LRRK2 expression.
Results: Differences were observed in LRRK2 levels between treatment groups, however they were found to be insignificant 3-weeks post-injection. For future research, larger cohorts and measuring LRRK2 levels at later time-points should be considered.
Keywords
Parkinson's Disease
ELISA
Lab-work
Subject
Biomedical Sciences
Date Available
June 10, 2024
Type
Poster
Course
BMS 4650 Capstone in Biomedical Sciences
Instructor
Polissidis, Alexia
Session
Poster Display
Location
Student Lounge
The American College of Greece, 6, Γραβιάς, Agia Paraskevi, Municipality of Agia Paraskevi, Regional Unit of North Athens, Attica, 150 01, Greece
